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Miroslav Zeidler1, Vladislav Čurn2 |
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1Palacký University Olomouc,
Faculty of Natural Sciences, Department of Ecology and Environmental Sciences,
Třída Svobody 26, Olomouc 77146, Czech Republic, e-mail address: zeidler@prfnw.upol.cz
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Abstract: The genetic diversity of re-established population of endangered
species Allium angulosum was tested
as a one part of rescue program. Founder individuals were picked in Chropyně
- Záříčí area (North Moravia, Czech republic) and new population was set in
Protected Landscape Area Litovelské Pomoraví (North Moravia, Czech republic).
The task was whether the newly founded population was made by representative
individuals to cover (include) the genetic variability of source(mother)
population. Items were tested with variability assay of six isozyme systems
(G-6-PDH, AAT, PGM, EST, ACP, PGI) using discontinuous polyacrylamide gel
electrophoresis (PAGE). The method stated relatively sufficient level of
variability on condition that new population would be raised to prevent
genetic changes. Application of more tests checking the genetic diversity
within population could be useful during reintroduction and management of
endangered plant species. |
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Key words:
isozymes, PAGE, Allium
angulosum, genetic diversity, conservation |